Composite

Part:BBa_K3406014:Design

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-10-27)


H1N1 virus simulation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 80
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to simulate the specific recognition of the detection, we selected a characteristic sequence from the viral genome. Besides, we added T7 promoter to the 5 'end of the gene to control the transcription of viral RNA. Because we don't need to get the translated product, so we didn't add RBS to the 5 'end of the gene.


Source

NCBI NC_007366

References